Molecular cloning of haploid germ cell‐specific tektin cDNA and analysis of the protein in mouse testis

FEBS Letters

Nishimune, Yoshitake²  Fujii, Takayuki¹  Tamura, Kazutoshi¹  Nojima, Hiroshi¹  Kaneko, Yoshioki²  Tanaka, Hiromitsu²  Iguchi, Naoko² 

[1] Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565-0871, Japan;Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565-0871, Japan

关键词: Mouse;    Testis;    Tektin;    cDNA library;    Sperm;   

DOI  :  10.1016/S0014-5793(99)00967-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tektins are a class of proteins that form filamentous polymers in the walls of ciliary and flagellar microtubules. We report here the molecular cloning of a new member of the tektin family, tektin-t, identified from a mouse haploid germ cell-specific cDNA library. Tektin-t mRNA encodes a protein of 430 deduced amino acids possessing RSNVELCRD, the conserved sequence of tektin family proteins. Western blotting showed a single band having a molecular weight of 86 kDa in the mouse testis. Immunohistochemistry of the testis showed that tektin-t is localized in the flagella of elongating spermatids from developmental step 15 to maturity.

【 授权许可】

Unknown