期刊论文详细信息
FEBS Letters
Molecular cloning of haploid germ cell‐specific tektin cDNA and analysis of the protein in mouse testis
Nishimune, Yoshitake2  Fujii, Takayuki1  Tamura, Kazutoshi1  Nojima, Hiroshi1  Kaneko, Yoshioki2  Tanaka, Hiromitsu2  Iguchi, Naoko2 
[1] Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565-0871, Japan;Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565-0871, Japan
关键词: Mouse;    Testis;    Tektin;    cDNA library;    Sperm;   
DOI  :  10.1016/S0014-5793(99)00967-9
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Tektins are a class of proteins that form filamentous polymers in the walls of ciliary and flagellar microtubules. We report here the molecular cloning of a new member of the tektin family, tektin-t, identified from a mouse haploid germ cell-specific cDNA library. Tektin-t mRNA encodes a protein of 430 deduced amino acids possessing RSNVELCRD, the conserved sequence of tektin family proteins. Western blotting showed a single band having a molecular weight of 86 kDa in the mouse testis. Immunohistochemistry of the testis showed that tektin-t is localized in the flagella of elongating spermatids from developmental step 15 to maturity.

【 授权许可】

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