Characterization of altered regulation variants of dinitrogenase reductase‐activating glycohydrolase from Rhodospirillum rubrum

FEBS Letters

Zhang, Yaoping¹  Roberts, Gary P¹  Kim, Kitai¹ 

[1] Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA

关键词: Nitrogen fixation;    Posttranslational regulation;    Adenosine diphosphate-ribosylation;    Dinitrogenase reductase-activating glycohydrolase;    Rhodospirillum rubrum;   

DOI  :  10.1016/S0014-5793(04)00031-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Rhodospirillum rubrum, nitrogenase activity is subject to posttranslational regulation through the adenosine diphosphate (ADP)-ribosylation of dinitrogenase reductase by dinitrogenase reductase ADP-ribosyltransferase (DRAT) and dinitrogenase reductase-activating glycohydrolase (DRAG). To study the posttranslational regulation of DRAG, its gene was mutagenized and colonies screened for altered DRAG regulation. Three different mutants were found and the DRAG variants displayed different biochemical properties including an altered affinity for divalent metal ions. Taken together, the results suggest that the site involved in regulation is physically near the metal binding site of DRAG.

【 授权许可】

Unknown