Volume‐regulated Cl− channels in human pleural mesothelioma cells

FEBS Letters

Meyer, Giuliano²  Manfredi, Rosangela²  Bazzini, Claudia²  Dossena, Silvia²  Guizzardi, Fabiana²  Bottà, Guido²  Catania, Anna¹  Garavaglia, Lisa²  Rodighiero, Simona²  Sironi, Chiara²  Paulmichl, Markus²  Bertocchi, Cristina² 

[1] Division of Internal Medicine, Padiglione Granelli, Ospedale Maggiore di Milano IRCCS, Via F. Sforza 35, 20122 Milan, Italy;Department of Biomolecular Sciences and Biotechnologies, University of Milan, Via Celoria 26, I-20133 Milan, Italy

关键词: Cl− channel;    Regulatory volume decrease;    ICln;    Adenosine 5′-triphosphate inhibition;    Pleural mesothelium;    Immunocytochemistry;   

DOI  :  10.1016/S0014-5793(04)00020-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Anion channels in human mesothelial and mesothelioma cell lines were characterized by patch-clamp and biomolecular approaches. We found an outwardly rectifying anionic current which was inactivated at positive voltages and inhibited by extracellular adenosine 5′-triphosphate (ATP). Mesothelial and mesothelioma cells behaved differently concerning current inactivation properties. Inactivation is more pronounced and has a steeper onset in mesothelial cells. Different reversal potentials, in asymmetrical Cl⁻ solutions, that could be attributed to a different selectivity of the channel, have been observed in the two cell lines. Mesothelioma cell single-channel analysis indicates that the number of the same active anion channel (3–4 pS) increased under hypoosmotic conditions. Immunocytochemistry experiments showed the presence of ICln protein in the cytosol and in the plasma membrane. Western blot analysis revealed an increase of ICln in the membrane under hypotonic conditions, an event possibly related to the activation of Cl⁻ channels.

【 授权许可】

Unknown