EGF Stimulates IClswell by a Redistribution of Proteins Involved in Cell Volume Regulation

Cellular Physiology and Biochemistry

关键词: EGF;    IClswell;    ICln;    HSPC038;    RVD;   

DOI  :  10.1159/000335851

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background ICln is a multifunctional protein involved in the generation of chloride currents activated during regulatory volume decrease (RVD) after cell swelling (ICl_swell). Growth factor receptors play a key role in different cellular processes and epidermal growth factor (EGF) regulates swelling-activated chloride permeability. Aim We set out to investigate if the EGF-induced upregulation of ICl_swell could be explained by a rearrangement of ICln subcellular distribution and interaction with its molecular partners. Methods NIH-3T3 fibroblasts were serum-deprived for 24 hours and stimulated with EGF (40 ng/ml) for 30 minutes. ICl_swell activation, ICln distribution and interaction with its molecular partner HSPC038 were assessed by whole cell patch clamp and fluorescence resonance energy transfer (FRET). Results EGF treatment significantly enhanced the direct molecular interaction between ICln and HSPC038 and also resulted in an increase of ICln and HSPC038 association with the plasma membrane. Importantly, these events are associated with a significant increase of ICl_swell. Conclusions The present data indicate that EGF might exert its role in the modulation of volume-sensitive chloride currents in part through activation and translocation of ICln and HSPC038 to the plasma membrane.

【 授权许可】

CC BY-NC-ND