The C‐terminal region as a modulator of rNav1.7 and rNav1.8 expression levels

FEBS Letters

Chahine, Mohamed¹  Acharfi, Said¹  Vijayaragavan, Kausalia¹ 

[1] Laval University, Department of Medicine, and Laval Hospital, Research Centre, 2725 chemin Sainte-Foy, Sainte-Foy, QC, Canada G1V 4G5

关键词: Voltage-gated Na+ channel;    C-terminus;    rNav1.7;    rNav1.8;    tsA201;    Dorsal root ganglion;    ER;    endoplasmic reticulum;    TTX;    tetrodotoxin;   

DOI  :  10.1016/S0014-5793(04)00019-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mammalian cells poorly express rNa_v1.8 channels. In contrast, rNa_v1.7 dorsal root ganglion channels have 90-fold higher peak Na⁺ current densities. We investigated the role of rNa_v1.7 and rNa_v1.8 carboxy-termini in modulating the expression of rNa_v1.7 and rNa_v1.8 channels in tsA201 cells. Mutations in the ubiquitination site of the C-terminus did not improve rNa_v1.8 current levels. However, rNa_v1.8 chimeras containing the entire or the proximal portion of the rNa_v1.7 C-terminus expressed 3.2-fold and 4.8-fold higher peak current densities, respectively, than parent rNa_v1.8 channels. We conclude that the two Na⁺ channels may have different endoplasmic reticulum processing signals.

【 授权许可】

Unknown