Ternary complex formation between DNA‐adenovirus core protein VII and TAF‐Iβ/SET, an acidic molecular chaperone

FEBS Letters

Gyurcsik, Bela¹  Nagata, Kyosuke¹  Okuwaki, Mitsuru¹  Haruki, Hirohito¹ 

[1] Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

关键词: Adenovirus;    Chromatin immunoprecipitation;    Histone chaperone;    Replication;    Transcription;   

DOI  :  10.1016/S0014-5793(03)01336-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The adenovirus (Ad) genome complexed with viral core proteins designated Ad core is the template for transcription of early genes and the first round of replication in Ad-infected cells. A cellular protein designated template-activating factor-I (TAF-I) is found to be involved in remodeling of the Ad core in vitro. Here we found that TAF-I interacts with the Ad DNA through core protein VII in infected cells in early phases of infection. In vitro binding assays using recombinant proteins showed that TAF-I forms ternary complexes with DNA–protein VII complexes.

【 授权许可】

Unknown