Characterization of human circulating TIG2 as a ligand for the orphan receptor ChemR23

FEBS Letters

John, H.¹  Forssmann, W.G.¹  Meyer, M.¹  Spodsberg, N.¹  Schleuder, D.³  Meder, W.¹  Richter, R.¹  Busmann, A.¹  Wendland, M.²  Kutzleb, C.¹ 

[1] IPF PharmaCeuticals GmbH, Feodor-Lynen-Str. 31, D-30625 Hannover, Germany;Pharis Biotec GmbH, Feodor-Lynen-Str. 31, D-30625 Hannover, Germany;Applied Biosystems, Frankfurter Str. 129 B, D-64293 Darmstadt, Germany

关键词: Ligand;    Orphan receptor;    ChemR23;    TIG2;    GPCR;    GPCR;    G-protein coupled receptor;    HPLC;    high performance liquid chromatography;    TFA;    trifluoroacetic acid;    PCR;    polymerase chain reaction;    MALDI-TOF-MS;    matrix-assisted laser desorption/ionization time-of-flight mass spectrometry;    CHO;    Chinese hamster ovary;   

DOI  :  10.1016/S0014-5793(03)01312-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The orphan receptor ChemR23 is a G-protein coupled receptor (GPCR) with homology to neuropeptide and chemoattractant receptors. Tazarotene, a synthetic retinoid activating retinoic acid receptor (RAR), up-regulates tazarotene-induced gene-2 (TIG2). The function and molecular target of this protein are now described. By means of reverse pharmacology screening using a peptide library generated from human hemofiltrate, we have isolated and identified TIG2 as the natural ligand of ChemR23 and report the specific molecular form of the bioactive, circulating TIG2, representing the amino-acid residues 21 to 154 of the 163 amino acid-containing prepropeptide. Based on the expression pattern of ChemR23 and TIG2, the physiological role in bone development, immune and inflammatory responses and the maintenance of skin is now being investigated.

【 授权许可】

Unknown