Essential role of the low density lipoprotein receptor‐related protein in vascular smooth muscle cell migration

FEBS Letters

Bu, Guojun¹  Li, Yonghe¹  Lu, Wenyan¹ 

[1] Department of Pediatrics, Washington University School of Medicine and St. Louis Children's Hospital, St. Louis, MO 63110, USA

关键词: Low density lipoprotein receptor-related protein;    Short interfering RNA;    Smooth muscle cell;    Ligand degradation;    Cell migration;    α2M;    α2-macroglobulin;    LDLR;    low density lipoprotein receptor;    LRP;    low density lipoprotein receptor-related protein;    PAI-1;    plasminogen activator inhibitor type 1;    PDGF;    platelet-derived growth factor;    RAP;    receptor-associated protein;    siRNA;    short interfering RNA;    uPA;    urokinase plasminogen activator;   

DOI  :  10.1016/S0014-5793(03)01272-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The low density lipoprotein receptor-related protein (LRP) is a multifunctional cell surface receptor highly expressed in human aortic smooth muscle cells. In the present study, we used the short interfering RNA (siRNA) technique to explore the role of LRP in smooth muscle cell migration. We identified an LRP-specific siRNA that selective silences LRP expression in human aortic smooth muscle cells. As a consequence, LRP-mediated ligand degradation was significantly reduced. More important, we found that platelet-derived growth factor-dependent cell migration was inhibited in cells transfected with LRP siRNA. These results demonstrate an important role of LRP in smooth muscle cell migration.

【 授权许可】

Unknown