Characterization of carbohydrates in the α2‐macroglobulin receptor

FEBS Letters

Jensen, Poul H.²  Gliemann, Jørgen²  Ørntoft, Torben¹ 

[1]Department of Clinical Chemistry, University Hospital of Aarhus, DK-8000 Aarhus C, Denmark

[2]Institute of Medical Biochemistry, University of Aarhus, DK-8000 Aarhus C, Denmark

关键词: α2-Macroglobulin receptor;    Low density lipoprotein receptor-related protein;    Glycosylation;    Lectin;    α2M;    α2-macroglobulin;    α2MR;    α2-macroglobulin receptor;    α2MRAP;    α2-macroglobulin receptor-associated protein;    LRP;    low density lipoprotein receptor-related protein;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;    O-glycanase;    endo-α-N-acetylgalactosaminidase;    Endo-H;    endo-β-N-acetylglucosaminidase H;    PNGase-F;    peptide N-glycohydrolase F;    Lectins (agglutinins);    ACA;    amarantus caudatus;    PNA;    arachis hypogeae;    BS-I;    bandeiraea simplicifolia I;    ConA;    canavalia ensiformis;    DSA;    datura stramonium;    ECG;    erythrina crista galli;    GNA;    galanthus nivalis;    LCA;    lens culinaris;    MAA;    maackia amurensis;    SNA;    sambucus nigra;    WGA;    triticum vulgaris;    PWM;    phytolacca americana;    VVA;    vicia villosa;   

DOI  :  10.1016/0014-5793(92)80879-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Carbohydrates were characterized in the human placental α₂-macroglobulin receptor and its associated protein. Carbohydrates, largely N-linked, contributed to about 18% of the size of the receptor α-chain and to about 25% of the β-chain. The 40 kDa receptor-associated Protein also contained carbohydrate. The α- and β-chains contained a wide variety of carbohydrates as judged by binding of lectins. Monosaceharide-competing inhibition of α₂M-methylamine binding by WGA suggested a functional significance of sugars in binding of ligands to the α-chain.

【 授权许可】

Unknown