Coordinate expression of the α and β subunits of heterotrimeric G proteins involves regulation of protein degradation in CHO cells

FEBS Letters

Juhnn, Yong-Sung²  Lee, Yun-Il²  Cho, Du-Hyong²  Cho, Chin-Ho²  Kwak, Sahng-June¹  Kim, So-Young²  Seo, MiRan² 

[1] Department of Biochemistry, College of Medicine, Dankook University, Chonan 330-714, South Korea;Department of Biochemistry and Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, South Korea

关键词: Heterotrimeric GTP-binding protein;    G protein α subunit;    G protein βγ subunit;    Coordinate expression;    Protein degradation;    Proteasome;    CHO;    Chinese hamster ovary;    G protein;    heterotrimeric GTP-binding regulatory protein;    Gα;    α subunit of G protein;    Gαs;    α subunit of stimulatory G protein;    Gβ;    β subunit of G protein;    Gβγ;    βγ dimer of G protein;    HA;    hemagglutinin;   

DOI  :  10.1016/S0014-5793(03)01267-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Individual cell types express a characteristic balance between heterotrimeric G protein α and βγ subunits, but little is known about the regulatory mechanism. We systemically examined the regulatory mechanism in CHO cells. We found that expression of Gαs, Gαi2, and Gαq proteins increased in direct proportion to the increase of Gβ1γ2 overexpressed transiently. Expression of Gβ protein also increased following overexpression of Gαs, Gαi2, and Gαq. The Gβγ overexpression stimulated degradation of Gβ in contrast to reduction of Gαs degradation. We conclude that coordinate expression of the G protein subunits involves regulation of protein degradation via proteasome in CHO cells.

【 授权许可】

Unknown