Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover

Biotechnology for Biofuels

Shihui Yang¹  Fengxian Hu²  Yanqing He²  Jie Bao²  Qiuqiang Gao²  Jian Zhang²  Xia Wang³  Xia Yi⁴  Gen Zou⁵  Lei Zhang⁵  Zhihua Zhou⁵ 

[1] 0000 0001 0727 9022, grid.34418.3a, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, 430062, Wuhan, China;0000 0001 2163 4895, grid.28056.39, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China;0000 0001 2163 4895, grid.28056.39, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China;0000 0001 0727 9022, grid.34418.3a, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, 430062, Wuhan, China;0000 0001 2163 4895, grid.28056.39, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China;grid.440811.8, Jiangxi Provincial Laboratory of Systems Biomedicine, Jiujiang University, 17 Lufeng Road, 332000, Jiujiang, China;0000000119573309, grid.9227.e, CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032, Shanghai, China;

关键词: Biodetoxification;    Amorphotheca resinae;    Heterozygous diploid;    Gene pair;    Coordinate expression;   

DOI  :  10.1186/s13068-019-1466-z

来源: publisher

【 摘 要 】

BackgroundFast, complete, and ultimate removal of inhibitory compounds derived from lignocellulose pretreatment is the prerequisite for efficient production of cellulosic ethanol and biochemicals. Biodetoxification is the most promising method for inhibitor removal by its unique advantages. The biodetoxification mechanisms of a unique diploid fungus responsible for highly efficient biodetoxification in solid-state culture was extensively investigated in the aspects of cellular structure, genome sequencing, transcriptome analysis, and practical biodetoxification.ResultsThe inborn heterozygous diploid structure of A. resinae ZN1 uniquely contributed to the enhancement of inhibitor tolerance and conversion. The co-expression of gene pairs contributed to the enhancement of the degradation of lignocellulose-derived model inhibitors. The ultimate inhibitors degradation pathways and sugar conservation were elucidated by microbial degradation experimentation as well as the genomic and transcriptomic sequencing analysis.ConclusionsThe finding of the heterozygous diploid structure in A. resinae ZN1 on biodetoxification took the first insight into the global overview of biodetoxification mechanism of lignocellulose-derived inhibitors. This study provided a unique and practical biodetoxification biocatalyst of inhibitor compounds for lignocellulose biorefinery processing, as well as the synthetic biology tools on biodetoxification of biorefinery fermenting strains.

【 授权许可】

CC BY