Arginine kinase overexpression improves Trypanosoma cruzi survival capability

FEBS Letters

Ivaldi, Soledad¹  Silber, Ariel M²  Alves, Maria Júlia M²  Pereira, Claudio A¹  Torres, Héctor N¹  Alonso, Guillermo D¹  Flawiá, Mirtha M¹ 

[1] Instituto de Investigaciones en Ingenierı́a Genética y Biologı́a Molecular, Consejo Nacional de Investigaciones Cientı́ficas y Técnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina;Laboratório de Bioquı́mica de Parasitas, Instituto de Quı́mica, Universidade de São Paulo, São Paulo, Brazil

关键词: Arginine kinase;    Phosphoarginine;    Phosphagen;    Guanidino kinase;    Trypanosoma cruzi;    LIT;    liver infusion tryptose;    pTREX-AK;    pTREX vector containing the arginine kinase gene;    pTREX-GFP;    pTREX vector containing the green fluorescent protein gene;    TAU-3AG;    triatomine artificial urine medium supplemented with proline;    aspartate;    glutamate and glucose;    UE;    μmol min−1 mg−1;   

DOI  :  10.1016/S0014-5793(03)01171-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Arginine kinase catalyzes the reversible transphosphorylation between adenosine diphosphate (ADP) and phosphoarginine, which is involved in temporal and spatial adenosine triphosphate (ATP) buffering. Here we demonstrate that the homologous overexpression of the Trypanosoma cruzi arginine kinase improves the ability of the transfectant cells to grow and resist nutritional and pH stress conditions. The stable transfected parasites showed an increased cell density since day 10 of culture, when the carbon sources became scarce, which resulted 2.5-fold higher than the control group on day 28. Additional stress conditions were also tested. We propose that arginine kinase is involved in the adaptation of the parasite to environmental changes.

【 授权许可】

Unknown