Spectral tuning of obelin bioluminescence by mutations of Trp92

FEBS Letters

Lee, John²  Stepanyuk, Galina A¹  Vysotski, Eugene S¹  Frank, Ludmila A¹  Malikova, Natalia P¹  Markova, Svetlana V¹ 

[1] Photobiology Lab, Institute of Biophysics, Russian Academy of Sciences Siberian Branch, Krasnoyarsk 660036, Russia;Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA

关键词: Photoprotein;    Aequorin;    Calcium;    Fluorescence;   

DOI  :  10.1016/S0014-5793(03)01166-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Ca²⁺-regulated photoprotein obelin was substituted at Trp92 by His, Lys, Glu, and Arg. All mutants fold into stable conformations and produce bimodal bioluminescence spectra with enhanced contribution from a violet emission. The W92R mutant has an almost monomodal bioluminescence (λ _max=390 nm) and monomodal fluorescence (λ _max=425 nm) of the product. Results are interpreted by an excited state proton transfer mechanism involving the substituent side group and His22 in the binding cavity.

【 授权许可】

Unknown