An assessment of ADAMs in bone cells: absence of TACE activity prevents osteoclast recruitment and the formation of the marrow cavity in developing long bones

FEBS Letters

del Carmen Ovejero, Maria¹  Lenhard, Thomas R¹  Boissy, Patrice¹  Kirkegaard, Tove¹  Peschon, Jacques J²  Delaissé, Jean-Marie¹  Black, Roy A² 

[1] Nordic Bioscience/CCBR, Herlev Hovedgade 207, 2730 Herlev, Denmark;Amgen, Seattle, WA 98101, USA

关键词: Bone;    Osteoclast recruitment;    A disintegrin and metalloprotease domain;    Metalloprotease;    TNFα converting enzyme;    ADAM;    a disintegrin and metalloprotease domain;    MMP;    matrix metalloproteinase;    TRAP;    tartrate resistant acid phosphatase;    ECM;    extracellular matrix;    TACE;    TNFα converting enzyme;   

DOI  :  10.1016/S0014-5793(03)01022-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

ADAMs (A Disintegrin And Metalloprotease domain) are metalloprotease–disintegrin proteins that have been implicated in cell adhesion, protein ectodomain shedding, matrix protein degradation and cell fusion. Since such events are critical for bone resorption and osteoclast recruitment, we investigated whether they require ADAMs. We report here which ADAMs we have identified in bone cells, as well as our analysis of the generation, migration and resorptive activity of osteoclasts in developing metatarsals of mouse embryos lacking catalytically active ADAM 17 [TNFα converting enzyme (TACE)]. The absence of TACE activity still allowed the generation of cells showing an osteoclastic phenotype, but prevented their migration into the core of the diaphysis and the subsequent formation of marrow cavity. This suggests a role of TACE in the recruitment of osteoclasts to future resorption sites.

【 授权许可】

Unknown