Conditional expression of exogenous Bcl‐XS triggers apoptosis in human melanoma cells in vitro and delays growth of melanoma xenografts

FEBS Letters

Eberle, Jürgen¹  Fecker, Lothar F¹  Orfanos, Constantin E¹  Hossini, Amir M¹  Geilen, Christoph C¹ 

[1] Charité - Universitätsmedizin Berlin, Campus Benjamin Franklin, Department of Dermatology, Fabeckstrasse 60–62, 14195 Berlin, Germany

关键词: Bcl-X;    Melanoma;    Apoptosis;    Transfection;    Chemotherapeutics;    NHM;    normal human melanocytes;    RT-PCR;    reverse transcriptase-polymerase chain reaction;    PAGE;    polyacrylamide gel electrophoresis;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(03)01017-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Bcl-2-related proteins Bcl-X_L and Bcl-X_S represent alternative splice products and exert opposite activities in the control of apoptosis, but their significance for melanoma is not yet clear. Applying the tetracycline-inducible expression system Tet-On, we found overexpression of Bcl-X_S by itself sufficient to induce apoptosis in vitro in stably transfected human melanoma cell lines. Combination with proapoptotic agents such as etoposide, pamidronate, and ceramide resulted in additive proapoptotic effects, whereas Bcl-X_L protected from apoptosis caused via CD95/Fas stimulation. In nude mice growth of melanoma xenotransplants derived from stably transfected cells was significantly reduced after induction of Bcl-X_S by doxycycline. Our results indicate that Bcl-X proteins are of major importance for control of apoptosis in malignant melanoma.

【 授权许可】

Unknown