Functional activities and cellular localization of the ezrin, radixin, moesin (ERM) and RING zinc finger domains in MIR

FEBS Letters

Lindholm, Dan¹  Johansson, Cecilia¹  Bornhauser, Beat C¹ 

[1] Department of Neuroscience, Neurobiology, Uppsala University, Biomedical Centre, Box 587, S-751 23 Uppsala, Sweden

关键词: Myosin regulatory light chain interacting protein;    Ezrin;    radixin;    moesin (ERM) proteins;    Neurite outgrowth;    Ubiquitination;    MIR;    myosin regulatory light chain interacting protein;    XIAP;    X-linked inhibitor of apoptosis protein;    MRLC;    myosin regulatory light chain;   

DOI  :  10.1016/S0014-5793(03)01010-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Myosin regulatory light chain interacting protein (MIR) belongs to the ezrin, radixin, moesin (ERM) family of proteins involved in membrane cytoskeleton interactions and cell dynamics. MIR contains, beside the ERM domain, a RING zinc finger region. Immunocytochemistry showed that full-length MIR and the subdomains localize differently in cells. Cell fractionation revealed a similar distribution of full-length MIR and the RING domain protein in the Triton X-100-insoluble fraction. The neurite outgrowth inhibitory activity of MIR was attributed to the RING domain. MIR levels were controlled in the cells depending on the intact RING domain and proteasome activity. The dynamic regulation of MIR contributes to its effects on neurite outgrowth and cell motility.

【 授权许可】

Unknown