pkn22 (alr2502) encoding a putative Ser/Thr kinase in the cyanobacterium Anabaena sp. PCC 7120 is induced by both iron starvation and oxidative stress and regulates the expression of isiA

FEBS Letters

Zhang, Cheng-Cai¹  Xu, Wen-Liang²  Liu, Yong-Ding²  Jeanjean, Robert¹ 

[1] Laboratoire de Chimie Bactérienne, IBSM-CNRS, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France;Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, PR China

关键词: Signal transduction;    Iron;    Photosynthesis;    Protein phosphorylation;    Sp;    spectinomycin;    Sm;    streptomycin;    MV;    methyl viologen;    PS;    photosystem;   

DOI  :  10.1016/S0014-5793(03)01019-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In cyanobacteria, the isiA gene is required for cell adaptation to oxidative damage caused by the absence of iron. We show here that a putative Ser/Thr kinase gene, pkn22 (alr2052), is activated by iron deficiency and oxidative damage in Anabaena sp. PCC 7120. A pkn22 insertion mutant is unable to grow when iron is limiting. pkn22 regulates the expression of isiA (encoding CP43′), but not of isiB (encoding flavodoxin) and psbC (CP43). Fluorescence measurement at 77 K reveals the absence of the typical signature of CP43′ associated with photosystem I in the mutant under iron-limiting conditions. We propose that Pkn22 is required for the function of isiA/CP43′ and constitutes a regulatory element necessary for stress response.

【 授权许可】

Unknown