Regulation of glycogen synthase kinase 3 in human platelets: a possible role in platelet function?

FEBS Letters

Gibbins, Jonathan M.¹  Fry, Michael J.¹  Barry, Fiona A.¹  Graham, Gwenda J.¹ 

[1] School of Animal and Microbial Sciences, The University of Reading, Whiteknights, PO Box 228 Reading RG6 6AJ, UK

关键词: Platelet;    Platelet activation;    Platelet aggregation;    Glycogen synthase kinase 3;    Phosphoinositide 3-kinase;    Protein kinase B;    GSK3;    glycogen synthase kinase 3;    GPVI;    glycoprotein VI;    PI3K;    phosphoinositide 3-kinase;    PKB;    protein kinase B;    DMSO;    dimethyl sulphoxide;   

DOI  :  10.1016/S0014-5793(03)01015-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study we show that both glycogen synthase kinase 3 (GSK3) isoforms, GSK3α and GSK3β, are present in human platelets and are phosphorylated on Ser²¹ and Ser⁹, respectively, in platelets stimulated with collagen, convulxin and thrombin. Phosphorylation of GSK3α/β was dependent on phosphoinositide 3-kinase (PI3K) activity and independent of platelet aggregation, and correlated with a decrease in GSK3 activity that was preserved by pre-incubating platelets with PI3K inhibitor LY294002. Three structurally distinct GSK3 inhibitors, lithium, SB415286 and TDZD-8, were found to inhibit platelet aggregation. This implicates GSK3 as a potential regulator of platelet function.

【 授权许可】

Unknown