B cell receptor‐mediated nuclear fragmentation proceeds in WEHI 231 cells in the absence of detectable DEVDase and FRase activity

FEBS Letters

Turk, Boris¹  Mlinaric-Rascan, Irena² 

[1] Department of Biochemistry and Molecular Biology, Josef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia;Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia

关键词: Caspase;    Cathepsin;    B cell receptor;    Apoptosis;    Inhibitor;    Ac-DEVD.AMC;    acetyl-Asp-Glu-Val-Asp-AMC;    AFC;    7-amino-4-trifluoromethyl coumarin;    AMC;    7-amino-4-methyl coumarin;    DMSO;    dimethylsulfoxide;    fmk;    fluoromethyl ketone;    z-VAD.fmk;    benzyloxycarbonyl-Val-Ala-Asp.fmk;    z-FR.AFC;    benzyloxycarbonyl-Phe-Arg-AFC;    E-64d;    (2S;    3S)-trans-epoxysuccinyl-leucylamido-3-methyl-butane ethyl ester;   

DOI  :  10.1016/S0014-5793(03)00966-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Crosslinking of the WEHI 231 lymphoma B cell receptor (BCR) leads to growth arrest followed by apoptosis. In a study of the role of lysosomal cysteine proteinases in BCR-mediated apoptosis we provide evidence that commitment to apoptosis correlates with a time-dependent increase in caspase and cathepsin activities. We also show that activation of cathepsins is a caspase-independent process, and caspase cascade activation is independent of lysosomal endopeptidases. BCR-induced nuclear fragmentation was not prevented, but rather delayed in the absence of detectable caspase and cathepsin activities, suggesting that BCR-driven apoptosis of these cells may use an alternative proteolytic mechanism independent of caspases and cathepsins.

【 授权许可】

Unknown