Inhibition of preadipocyte proliferation by mitochondrial reactive oxygen species

FEBS Letters

Carrière, Audrey¹  Fernandez, Yvette¹  Rigoulet, Michel²  Pénicaud, Luc¹  Casteilla, Louis¹ 

[1] UMR 5018 CNRS-UPS, IFR31, Bât. L1, CHU Rangueil, 31059 Toulouse Cedex 49, France;IBGC-CNRS, 1 rue Camille Saint-Saëns, 33077 Bordeaux, France

关键词: White preadipocyte;    Proliferation;    Mitochondrion;    Reactive oxygen species;    ROS;    reactive oxygen species;    DHR123;    dihydrorhodamine 123;    H2-DCFDA;    6-carboxy-2′;    7′-dichlorodihydrofluorescein diacetate;    diacetoxymethylester;    DMEM;    Dulbecco's modified Eagle's medium;    SVC;    stroma-vascular cells;    FBS;    fetal bovine serum;    CCCP;    carbonyl cyanide chlorophenylhydrazone;    BHA;    butylhydroxyanisole;   

DOI  :  10.1016/S0014-5793(03)00862-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Preadipocytes are present and can proliferate to increase fat mass throughout adult life. The importance of mitochondria in these cells has never been investigated, although we recently reported that mitochondrial oxidative metabolism is non-negligible in white preadipocytes. Mitochondrial reactive oxygen species generation is intimately associated with respiratory chain function. An increasing number of reports support their role as signalling molecules. The aim of this work was to study the effects of mitochondrial reactive oxygen species on proliferation of white preadipocytes. Rotenone and oligomycin, inhibitors of complex I and of ATP synthase respectively, increased H₂O₂ and inhibited cell growth of preadipocytes (without inducing necrosis or apoptosis). These effects were partly prevented by addition of radical scavengers. A chemical uncoupler had opposite effects on reactive oxygen species generation and cell growth. Propofol, which inhibits complex I but also scavenges free radicals, had effects similar to those of the uncoupler on both parameters. Thus, mitochondrial reactive oxygen species can influence development of adipose tissue by affecting the size of the white preadipocyte pool.

【 授权许可】

Unknown