Potassium uptake in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 mainly depends on a Ktr‐like system encoded by slr1509 (ntpJ)

FEBS Letters

Rögner, Matthias³  Berry, Stephan³  Elanskaya, Irina²  Esper, Berndt³  Teuber, Markus³  Hagemann, Martin¹  Karandashova, Inga² 

[1] FB Biowissenschaften, University Rostock, A.-Einstein-Str. 3, D-18057 Rostock, Germany;Department of Genetics, Faculty of Biology, Lomonossov Moscow State University, Moscow 119992, Russia;LS Biochemie der Pflanzen, Ruhr-University Bochum, Universitätsstr. 150, D-44780 Bochum, Germany

关键词: Ion transport;    Salt stress;    Sodium extrusion;    DBMIB;    2;    5-dibromo-6-isopropyl-3-methyl-1;    4-benzoquinone;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1′-dimethylurea;    Km;    kanamycin;    WT;    wild type;    Δμ(Na+);    sodium motive force;   

DOI  :  10.1016/S0014-5793(03)00729-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The molecular basis of potassium uptake in cyanobacteria has not been elucidated. However, genes known from other bacteria to encode potassium transporters can be identified in the genome of Synechocystis sp. strain PCC 6803. Mutants defective in kdpA and ntpJ were generated and characterized to address the role of the Kdp and KtrAB systems in this strain. KtrAB is crucial for K⁺ uptake, as the ΔntpJ mutant shows slowed growth, slowed potassium uptake kinetics, and increased salt sensitivity. The ΔkdpA mutant has the same phenotype as the wild type even at limiting potassium, but a ΔkdpAΔntpJ double mutant is not viable, indicating a role of Kdp for potassium uptake when the Ktr system is not functioning.

【 授权许可】

Unknown