Transferrins selectively cause ion efflux through bacterial and artificial membranes

FEBS Letters

Quiros, Luis M²  Aguilera, Oscar¹  Fierro, José F³ 

[1] Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands;Instituto Universitario de Oncologı́a del Principado de Asturias, Area de Fisiologı́a, Facultad de Medicina, Oviedo 33006, Spain;Departamento de Biologı́a Funcional, Facultad de Medicina, Universidad de Oviedo, Oviedo 33006, Spain

关键词: Transferrin;    Lactoferrin;    Ovotransferrin;    Serum transferrin;    Antibacterial mechanism;    Proton-motive force;    BHI;    brain heart infusion;    CD;    circular dichroism;    diS-C3-(5);    3;    3′-dipropylthiadicarbocyanine iodide;    pyranine;    8-hydroxypyrene-1;    3;    6-trisulfonic acid;    SUV;    small unilamellar vesicle;   

DOI  :  10.1016/S0014-5793(03)00719-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Serum transferrin, ovotransferrin and lactoferrin constitute the most notable members of the transferrin family. Among their multiple biological functions, they possess an important antibacterial activity. These proteins can permeate the Escherichia coli outer membrane, reaching the inner membrane where they selectively cause permeation of ions, resulting in dissipation of the electrical potential without affecting the pH gradient. Similar results were obtained using artificial liposomes, suggesting a direct action of the proteins on the lipid bilayer, which was mediated by detectable conformational changes in their structures.

【 授权许可】

Unknown