Late ischemic preconditioning of the myocardium alters the expression of genes involved in inflammatory response

FEBS Letters

Brändle, Marian¹  Kluxen, Franz-Werner¹  Ehring, Thomas¹  Frohme, Marcus²  Hentsch, Bernd¹  Zubakov, Dmitrij²  Hoheisel, Jörg D² 

[1] Merck KGaA, Biomedical Research CADS, 64271 Darmstadt, Germany;Functional Genome Analysis, German Cancer Research Center (DKFZ Heidelberg), Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany

关键词: Heart;    Preconditioning;    Gene expression;    Representational difference analysis;    Microarray;   

DOI  :  10.1016/S0014-5793(03)00667-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Myocardial ischemic preconditioning (IPC) is a potent endogenous mechanism of cardioprotection against ischemia–reperfusion injury. In this study we focused on the second phase of IPC as the most interesting in terms of therapeutic implementations. We aimed at the detection of genes, which are differentially expressed at 16 h after reperfusion. Preconditioning of canine myocardium was initiated by 5 min occlusion of the left anterior descending coronary artery with subsequent reperfusion. cDNA representational difference analysis in combination with microarray hybridization and reverse transcription polymerase chain reaction were used to reveal the changes in gene expression in canine hearts. We found that functionally related genes for tristetraproline (TTP), selectin E, matrix metalloproteinase 9, and tumor necrosis factor-α were highly upregulated at the late phase of IPC. The upregulation of TTP gene at the late phase of IPC, reported here for the first time, may represent a cardioprotective mechanism, which could be a promising perspective in clinical interventions against ischemia–reperfusion injuries of the heart.

【 授权许可】

Unknown