Cholesterol diet‐induced hyperlipidemia influences gene expression pattern of rat hearts: a DNA microarray study

FEBS Letters

Ónody, Annamária³  Kitajka, Klára¹  Hackler, László²  Giricz, Zoltán³  Zvara, Ágnes²  Csonka, Csaba³  Ferdinandy, Péter³  Nagy, Zsolt B.²  Puskás, László G.² 

[1] Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary;Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary;Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Dóm tér 9, H-6720 Szeged, Hungary

关键词: Cholesterol diet;    Hyperlipidemia;    Heart;    Gene expression;    Procollagen type III;    Chloride intracellular channel 4;    Tensin;    Hsp86;    Hsp105;    Farnesyltransferase;    Metallothionein;    NADH dehydrogenase;    CD81 antigen;    Catenin;   

DOI  :  10.1016/S0014-5793(04)00189-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To profile gene expression patterns involved in the direct myocardial effect of cholesterol-enriched diet-induced hyperlipidemia, we monitored global gene expression changes by DNA microarray analysis of 3200 genes in rat hearts. Twenty-six genes exhibited significant up-regulation and 25 showed down-regulation in hearts of rats fed a 2% cholesterol-enriched diet for 8 weeks as compared to age-matched controls. The expression changes of 12 selected genes were also assessed by real-time quantitative polymerase chain reaction. Genes with altered expression in the heart due to hyperlipidemia included procollagen type III, cofilin/destrin, tensin, transcription repressor p66, synaptic vesicle protein 2B, Hsp86, chaperonin subunit 5ϵ, metallothionein, glutathione S-transferase, protein kinase C inhibitor, ATP synthase subunit c, creatine kinase, chloride intracellular channel 4, NADH oxidoreductase and dehydrogenase, fibronectin receptor β chain, CD81 antigen, farnesyltransferase, calreticulin, disintegrin, p120 catenin, Smad7, etc. Although some of these genes have been suspected to be related to cardiovascular diseases, none of the genes has been previously shown to be involved in the mechanism of the cardiac effect of hyperlipidemia.

【 授权许可】

Unknown