Silencing of the gene coding for the ϵ subunit of DNA polymerase III slows down the growth rate of Escherichia coli populations

FEBS Letters

Hochkoeppler, Alejandro¹  Radeghieri, Annalisa¹  Reggiani, Luca¹  Gonzalez Vara y Rodriguez, Antonio¹  Cianchetta, Stefano¹  Stefan, Alessandra¹ 

[1] Department of Industrial Chemistry, University of Bologna, Viale Risorgimento 4, 40136 Bologna, Italy

关键词: Mutator strains;    Replication;    Antisense RNA;    DNA polymerase;    Escherichia coli;   

DOI  :  10.1016/S0014-5793(03)00604-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chromosome replication in Escherichia coli is accomplished by the multimeric enzyme DNA polymerase III; the relevance, in vivo, of the ϵ subunit (encoded by dnaQ) for processivity and fidelity of DNA polymerase III has been evaluated. To this aim, dnaQ has been conditionally silenced by means of in vivo expression of different antisense RNAs. Unexpectedly, the presence of the Shine–Dalgarno sequence is essential for the effectiveness of antisense constructs. Silencing of dnaQ induces a severe decrease in growth rate not paralleled by high mutation frequencies, suggesting that the ϵ subunit primarily affects the processivity of DNA polymerase III.

【 授权许可】

Unknown