Na+‐dependent fructose transport via rNaGLT1 in rat kidney

FEBS Letters

Masuda, Satohiro¹  Inui, Ken-ichi¹  Takeuchi, Daisuke¹  Okuda, Masahiro¹  Horiba, Naoshi¹  Ohnishi, Chiharu¹ 

[1]Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan

关键词: Rat Na+-dependent glucose transporter 1;    Fructose;    Kidney;    Brush-border membrane vesicle;    Na+-dependent;    rNaGLT1;    rat Na+-dependent glucose transporter 1;    αMeGlc;    α-methyl-D-glucopyranoside;    2-DG;    2-deoxy-D-glucose;   

DOI  :  10.1016/S0014-5793(03)00600-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We found a system of Na⁺-dependent uptake of fructose by rat renal brush-border membrane vesicles. It consisted of two saturable components, and was thought to involve at least two transporters. rNaGLT1, a novel glucose transporter in rat kidney, showed fructose uptake as well as α-methyl-D-glucopyranoside uptake by transfected HEK293 cells. The features of the lower affinity type of fructose transporter in the brush-border membranes, such as affinity and substrate recognition, were very comparable with those of rNaGLT1-transfected HEK293 cells. These results indicated that rNaGLT1 is a primary fructose transporter in rat renal brush-border membranes.

【 授权许可】

Unknown