Mesona Chinensis Benth extract prevents AGE formation and protein oxidation against fructose-induced protein glycation in vitro

BMC Complementary and Alternative Medicine

Charoonsri Chusak²  Thavaree Thilavech¹  Sirichai Adisakwattana² 

[1] Program in Biomedical Sciences, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand;Research Group of Herbal Medicine for Prevention and Therapeutic of Metabolic diseases, Chulalongkorn University, Bangkok 10330, Thailand

关键词: Advanced glycation end products;    Fructose;    Protein glycation;    Mesona chinensis;   

Others  :  1220193
DOI  :  10.1186/1472-6882-14-130

 received in 2013-10-14, accepted in 2014-03-31,  发布年份 2014

【 摘 要 】

Background

Mesona chinensis Benth (Chinese Mesona), an economically significant agricultural plant, is the most widely consumed as an herbal beverage in Southeast Asia and China. The objective of this study was to evaluate the inhibitory activity of Mesona chinensis (MC) extract on the formation of advanced glycation end products (AGEs) and protein oxidation in an in vitro model of fructose-mediated protein glycation.

Methods

The content of total polyphenolic compounds was measured by using Folin–Ciocalteu assay. Antiglycation activity was determined using the formation of AGE fluorescence intensity, N^ϵ-(carboxymethyl)lysine (CML), the level of fructosamine, and the formation of amyloid cross β-structure. The protein oxidation was examined using the level of protein carbonyl content and thiol group.

Results

Our results revealed that the content of total polyphenolic compound in MC extract was 212.4 ± 5.6 mg gallic acid equivalents/g dried extract. MC extract (0.25-1.00 mg/mL) significantly inhibited the formation of fluorescence AGEs in fructose-glycated bovine serum albumin (BSA) during 4 weeks of study. Furthermore, MC extract also decreased the level of N^ϵ-CML, fructosamine, and amyloid cross β-structure in fructose-glycated BSA. While the total thiol group was elevated and the protein carbonyl content was decreased in BSA incubated with fructose and MC extract.

Conclusions

The extract of MC inhibits fructose-mediated protein glycation and protein oxidation. This edible plant could be a natural rich source of antiglycation agent for preventing AGE-mediated diabetic complication.

【 授权许可】

   
2014 Adisakwattana et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Rondeau P, Bourdon E: The glycation of albumin: structural and functional impacts. Biochimie 2011, 93:645-658.
• [2]Ahmed N: Advanced glycation end products-role in pathology of diabetic complications. Diabetes Res Clin Pract 2005, 67:3-21.
• [3]Bierhaus A, Humpert PM, Morcos M, Wendt T, Chavakis T, Arnold B, Stern DM, Nawroth PP: Understanding RAGE, the receptor for advanced glycation end products. J Mol Med 2005, 83:876-886.
• [4]Freedman BI, Wuerth JP, Cartwright K, Bain RP, Dippe S, Hershon K, Mooradian AD, Spinowitz BS: Design and baseline characteristics for the aminoguanidine Clinical Trial in Overt Type 2 Diabetic Nephropathy (ACTION II). Control Clin Trials 1999, 20:493-510.
• [5]Bolton WK, Cattran DC, Williams ME, Adler SG, Appel GB, Cartwright K, Foiles PG, Freedman BI, Raskin P, Ratner RE, Spinowitz BS, Whittier FC, Wuerth JP, ACTION I Investigator Group: Randomized trial of an inhibitor of formation of advanced glycation end products in diabetic nephropathy. Am J Nephrol 2004, 24:32-40.
• [6]Dearlove RP, Greenspan P, Hartle DK, Swanson RB, Hargrove JL: Inhibition of protein glycation by extracts of culinary herbs and spices. J Med Food 2008, 11:275-281.
• [7]Jariyapamornkoon N, Yibchok-anun S, Adisakwattana S: Inhibition of advanced glycation end products by red grape skin extract and its antioxidant activity. BMC Complement Altern Med 2013, 13:171. BioMed Central Full Text
• [8]Adisakwattana S, Jiphimai P, Prutanopajai P, Chanathong B, Sapwarobol S, Ariyapitipan T: Evaluation of α-glucosidase, α-amylase and protein glycation inhibitory activities of edible plants. Int J Food Sci Nutr 2010, 61:295-305.
• [9]Saraswat M, Reddy PY, Muthenna P, Reddy GB: Prevention of non-enzymic glycation of proteins by dietary agents: prospects for alleviating diabetic complications. Br J Nutr 2009, 101:1714-1721.
• [10]Šebeková K, Somoza V: Dietary advanced glycation endproducts (AGEs) and their health effects–PRO. Mol Nutr Food Res 2007, 51:1079-1084.
• [11]Feng T, Gu ZB, Jin ZY: The research advances of the Mesona blume gum. China Food Addit 2005, 6:004.
• [12]Hailan S, Yingzhen H, Jingying C: Comparative analysis of amino acids content in Mesona chinensis from different producing areas. Chinese Wild Plant Resour 2011, 5:19.
• [13]Yuanping Z: Determination of total flavonoids in Mesona Chinensis by spectrophotometry. Acad Peri Farm Prod Process 2009, 6:33.
• [14]Mäkynen K, Jitsaardkul S, Tachasamran P, Sakai N, Puranachoti S, Nirojsinlapachai N, Chattapat V, Caengprasath N, Ngamukote S, Adisakwattana S: Cultivar variations in antioxidant and antihyperlipidemic properties of pomelo pulp (Citrus grandis [L.] Osbeck) in Thailand. Food Chem 2013, 139:735-743.
• [15]Sharma SD, Pandey BN, Mishra KP, Sivakami S: Amadori product and age formation during nonenzymatic glycosylation of bovine serum albumin in vitro. J Biochem Mol Biol Biophys 2002, 6:233-242.
• [16]Ardestani A, Yazdanparast R: Cyperus rotundus suppresses age formation and protein oxidation in a model of fructose-mediated protein glycoxidation. Int J Biol Macromol 2007, 41:572-578.
• [17]Bouma B, Kroon-Batenburg LM, Wu Y-P, Brünjes B, Posthuma G, Kranenburg O, de Groot PG, Voest EE, Gebbink MF: Glycation induces formation of amyloid cross-β structure in albumin. J Biol Chem 2003, 278:41810-41819.
• [18]Meeprom A, Sompong W, Chan CB, Adisakwattana S: Isoferulic acid, a new anti-glycation agent, inhibits fructose- and glucose-mediated protein glycation in vitro. Molecules 2013, 18:6439-6454.
• [19]Smith PR, Thornalley PJ: Mechanism of the degradation of non‒enzymatically glycated proteins under physiological conditions. Eur J Biochem 1992, 210:729-739.
• [20]Nagai R, Ikeda K, Higashi T, Sano H, Jinnouchi Y, Araki T, Horiuchi S: Hydroxyl radical mediates Nϵ-(Carboxymethyl) lysine formation from Amadori product. Biochem Biophys Res Commun 1997, 234:167-172.
• [21]Wu CH, Huang SM, Lin JA, Yen GC: Inhibition of advanced glycation endproduct formation by foodstuffs. Food Func 2011, 2:224-234.
• [22]Hinton D, Ames J: Site specificity of glycation and carboxymethylation of bovine serum albumin by fructose. Amino Acids 2006, 30:425-434.
• [23]Tappy L, Lê KA, Tran C, Paquot N: Fructose and metabolic diseases: new findings, new questions. Nutrition 2010, 26:1044-1049.
• [24]Schalkwijk CG, Stehouwer CD, van Hinsbergh VW: Fructose‒mediated non‒enzymatic glycation: sweet coupling or bad modification. Diabetes Metab Res Rev 2004, 20:369-382.
• [25]Sompong W, Meeprom A, Cheng H, Adisakwattana S: A comparative study of ferulic acid on different monosaccharide-mediated protein glycation and oxidative damage in bovine serum albumin. Molecule. 2013, 18:13886-13903.
• [26]Sakai M, Oimomi M, Kasuga M: Experimental studies on the role of fructose in the development of diabetic complications. Kobe J Med Sci 2002, 48:125-136.
• [27]Elosta A, Ghous T, Ahmed N: Natural products as anti-glycation agents: possible therapeutic potential for diabetic complications. Curr Diabetes Rev 2012, 8:92-108.
• [28]Wu CH, Yen GC: Inhibitory effect of naturally occurring flavonoids on the formation of advanced glycation endproducts. J Agric Food Chem 2005, 53:3167-3173.
• [29]Kusirisin W, Srichairatanakool S, Lerttrakarnnon P, Lailerd N, Suttajit M, Jaikang C, Chaiyasut C: Antioxidative activity, polyphenolic content and anti-glycation effect of some Thai medicinal plants traditionally used in diabetic patients. Med Chem 2009, 5:139-147.
• [30]Ho SC, Wu SP, Lin SM, Tang YL: Comparison of anti-glycation capacities of several herbal infusions with that of green tea. Food Chem 2010, 122:768-774.
• [31]Caengprasath N, Ngamukote S, Mäkynen K, Adisakwattana S: The protective effects of pomelo extract (Citrus Grandis L. Osbeck) against fructose-mediated protein oxidation and glycation. EXCLI J 2013, 12:491-502.
• [32]Marzban L, Verchere CB: The role of islet amyloid polypeptide in type 2 diabetes. Can J Diabetes 2004, 28:39-47.
• [33]Aćimović JM, Stanimirović BD, Mandić LM: The role of the thiol group in protein modification with methylglyoxal. J Serb Chem Soc 2009, 74:867-883.
• [34]Dalle-Donne I, Giustarini D, Colombo R, Rossi R, Milzani A: Protein carbonylation in human diseases. Trend Mol Med 2003, 9:169-176.
• [35]Hung CY, Yen GC: Antioxidant activity of phenolic compounds isolated from Mesona procumbens Hemsl. J Agric Food Chem 2002, 50:2993-2997.
• [36]Lai LS, Chou ST, Chao WW: Studies on the antioxidative activities of Hsian-tsao (Mesona procumbens Hemsl) leaf gum. J Agric Food Chem 2001, 49:963-968.
• [37]Chusak C, Thilavech T, Adisakwattana S: Consumption of Mesona Chinensis Benth attenuates postprandial glucose and improves antioxidant status induced by a high carbohydrate meal in overweight subjects. Am J Chinese Med 2014, 42:315-336.
• [38]Cervantes-Laurean D, Schramm DD, Jacobson EL, Halaweish I, Bruckner GG, Boissonneault GA: Inhibition of advanced glycation end product formation on collagen by rutin and its metabolites. J Nutr Biochem 2006, 17:531-540.