Identification of hepatic peroxisomal phospholipase A2 and characterization of arachidonic acid‐containing choline glycerophospholipids in hepatic peroxisomes

FEBS Letters

Yang, Jingyue²  Gross, Richard W²  Han, Xianlin¹ 

[1] Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, 660 South Euclid Avenue, Campus Box 8020, St. Louis, MO 63110, USA;Department of Chemistry, Washington University, Campus Box 1134, One Brookings Drive, St. Louis, MO 63130, USA

关键词: Phospholipase A2;    iPLA2γ;    Peroxisome;    Arachidonic acid;    Phospholipid;    Phosphatidylcholine;    ESI-MS;    electrospray ionization mass spectrometry;    MS/MS;    tandem mass spectrometry;    PtdCho;    phosphatidylcholine;    PtdEtn;    phosphatidylethanolamine;    PtdIns;    phosphatidylinositol;    PtdSer;    phosphatidylserine;    iPLA2γ;    calcium-independent phospholipase A2γ;   

DOI  :  10.1016/S0014-5793(03)00581-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recently, a sequence encoding a novel mammalian calcium-independent phospholipase A₂ (iPLA₂γ) was identified in the human genome and subsequently cloned and expressed in Sf9 insect cells. Unexpectedly, expression studies in recombinant systems demonstrated the usage of multiple translation initiation codons resulting in different polypeptides. Herein, we demonstrate that hepatic iPLA₂γ is localized to rat liver peroxisomes, possesses a molecular mass of 63 kDa and that peroxisomal membranes are highly enriched in arachidonic acid-containing phospholipids. Collectively, these results provide the first demonstration of iPLA₂γ in mammalian tissue and suggest the possibility that iPLA₂γ can contribute to lipid second messenger generation by hydrolysis of peroxisomal arachidonic acid-containing phospholipids.

【 授权许可】

Unknown