Peroxisomal‐microsomal communication in unsaturated fatty acid metabolism

FEBS Letters

Sprecher, Howard¹  Baykousheva, Svetla P.¹  Luthria, Devanand L.¹ 

[1] Department of Medical Biochemistry, College of Medicine, The Ohio State University, 1645 Neil Ave., 337B Hamilton Hall, Columbus, OH 43210, USA

关键词: Microsome;    Peroxisome;    Arachidonic acid;    7;    10;    13;    16-Docosatetraenoic acid;    β-Oxidation;    Acylation;   

DOI  :  10.1016/0014-5793(95)00565-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by β-oxidation of [1-¹⁴C]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity was due to microsomal contamination. The production of acid-soluble radioactivity from [1-¹⁴C]7,10,13,16–22:4, but not from [3-¹⁴C]7,10,13,16–22:4 was independent of 1-acyl-GPC, with and without microsomes. By comparing rates of peroxisomal β-oxidation with those for microsomal acylation, it was shown that the preferred metabolic fate of arachidonate, when added directly to incubations, or generated via β-oxidation, was esterification by microsomal 1-acyl-GPC acyltransferase, rather than continued peroxisomal β-oxidation.

【 授权许可】

Unknown