Neutrophil elastase up‐regulates human β‐defensin‐2 expression in human bronchial epithelial cells

FEBS Letters

Taggart, Clifford C.¹  McElvaney, Noel G.¹  Greene, Catherine M.¹  Griffin, Siobhan¹  O'Neill, Shane¹ 

[1] Respiratory Research Division, Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, Ireland

关键词: Human β-defensin-2;    Neutrophil elastase;    Innate immunity;    HBD-2;    human β-defensin-2;    IL-1β;    interleukin-1β;    LPS;    lipopolysaccharide;    NE;    neutrophil elastase;    NHBE;    normal human bronchial epithelial;    HBE;    human bronchial epithelial;    CF;    cystic fibrosis;    LSC;    laser scanning cytometry;    α1AT;    α1-antitrypsin;   

DOI  :  10.1016/S0014-5793(03)00577-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human β-defensin-2 (HBD-2) gene expression is induced by tumour necrosis factor-α, interleukin-1β and lipopolysaccharide. The objective of this study was to investigate the effect of neutrophil elastase (NE), a major pro-inflammatory protease, on HBD-2 expression. HBD-2 gene expression was assessed by reverse transcription polymerase chain reaction in the human bronchial epithelial cell line 16HBE14o− and primary normal human bronchial epithelial (NHBE) cells. Optimal HBD-2 expression was induced with 100 nM NE. Using a HBD-2-luciferase reporter construct, luciferase activity increased significantly in 16HBE14o− cells following incubation with NE. An increase in HBD-2 protein expression was observed in primary NHBE cells after incubation with NE as assessed by laser scanning cytometry. In conclusion, NE up-regulates HBD-2 expression in bronchial epithelial cells.

【 授权许可】

Unknown