Heme oxygenase‐1 induction by endogenous nitric oxide: influence of intracellular glutathione

FEBS Letters

Felley-Bosco, Emanuela¹  André, Muriel¹ 

[1] Institute of Pharmacology and Toxicology, Rue du Bugnon 27, 1005 Lausanne, Switzerland

关键词: Heme oxygenase-1;    Nitric oxide;    Glutathione;    Oxidative stress;    NO;    nitric oxide;    RNOS;    reactive nitrogen oxide species;    GSH;    glutathione;    HO-1;    heme oxygenase-1;    iNOS;    inducible nitric oxide synthase;    IPTG;    isopropyl-β-D-thiogalactoside;    BSO;    L-buthionine-[S;    R]-sulfoximine;    L-NMA;    N-methyl-L-arginine;    DETA/NO;    diethylenetriamine/nitric oxide;   

DOI  :  10.1016/S0014-5793(03)00576-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To investigate the influence of glutathione (GSH) on cellular effects of nitric oxide (NO) formation, human colon adenocarcinoma cells were transfected with a vector allowing controlled expression of inducible nitric oxide synthase (iNOS). Protein levels of oxidative stress-sensitive heme oxygenase-1 (HO-1) were analyzed in the presence or absence of GSH depletion using L-buthionine-[S,R]-sulfoximine and iNOS induction. While no effect was observed in the presence of iNOS activity alone, a synergistic effect on HO-1 expression was observed in the presence of iNOS expression and GSH depletion. This effect was prevented by addition of N-methyl-L-arginine. Therefore, targeting of endogenous NO may be modulated by intracellular GSH.

【 授权许可】

Unknown