Increased oxidative stress in the RAW 264.7 macrophage cell line is partially mediated via the S‐nitrosothiol‐induced inhibition of glutathione reductase

FEBS Letters

Butzer, Urwe¹  Gansauge, Susanne¹  Beger, Hans G¹  Weidenbach, Hans¹  Gansauge, Frank¹  Nussler, Andreas K¹ 

[1] Departments of General Surgery and Internal Medicine, University of Ulm, Ulm, Germany

关键词: S-nitrosothiol;    Glutathione;    Glutathione reductase;    Nitric oxide;    Oxidative stress;   

DOI  :  10.1016/S0014-5793(99)00139-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated whether endogenously or exogenously produced nitric oxide (NO) can inhibit cellular glutathione reductase (GR) via the formation of S-nitrosothiols to decrease cellular glutathione (GSH) and increase oxidative stress in RAW 264.7 cells. The specificity of this inhibition was demonstrated by addition of a NO-synthase inhibitor, and met- or oxyhemoglobin. Using isolated GR we found that only certain NO donors inhibit this enzyme via S-nitrosothiol. Furthermore, we found that cellular GSH decrease is paralleled by an increase of superoxide anion production. Our results show that the GR enzyme is a potential target of S-nitrosothiols to decrease cellular GSH levels and to induce oxidative stress in macrophages.

【 授权许可】

Unknown