Phasing of RecA monomers on quasi‐random DNA sequences

FEBS Letters

Bocharova, Tatjana N.²  Volodin, Alexander A.²  Camerini-Otero, R.Daniel¹  Smirnova, Elena A.² 

[1] Genetics and Biochemistry Branch, NIDDK, National Institutes of Health, Bethesda, MD 20892-1810, USA;Institute of Molecular Genetics of the Russian Academy of Sciences, Kurchatov sq., 2, 123182 Moscow, Russia

关键词: RecA protein;    Presynaptic complex;    RecA monomer positioning;    Homologous recombination;    DNA strand exchange;    ssDNA;    single-stranded DNA;    dsDNA;    double-stranded DNA;    DMS;    dimethyl sulfate;   

DOI  :  10.1016/S0014-5793(03)00572-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We show that some arbitrarily chosen DNA sequences have the ability to influence the positioning of RecA monomers in RecA–DNA complexes. The preferential phase of binding of RecA monomers is shown to depend on the DNA sequence and its nucleotide composition. A simple rearrangement of bases in a limited DNA stretch influences the phasing of RecA monomers. On the other hand, that some features of DNA sequences interfere with the phasing on specific DNA sites demonstrates the existence of mechanisms for both positive and negative regulation of phasing on natural DNAs. The possible role of phasing of RecA monomers on DNA is discussed.

【 授权许可】

Unknown