Periodicity in recA protein‐DNA complexes

FEBS Letters

Smirnova, Helen A¹  Bocharova, Tatjana N¹  Volodin, Alexander A¹ 

[1] Institute of Molecular Genetics of the Russian Academy of Sciences, Kurchatov sq., 123182 Moscow, Russia

关键词: RecA protein;    Fluorescent dye-labeled oligonucleotide;    DNA chemical modification;    ATP-S;    adenosine-5′-O-(3-thiotriphosphate);    TAMRA;    tetramethylrhodamine;    FAM;    5′-carboxyfluorescein;    PAG;    polyacrylamide gel;    DMS;    dimethylsulfate;    ss and ds;    single- and double-stranded;    respectively;    TEA;    triethanol acetate buffer;   

DOI  :  10.1016/S0014-5793(97)00367-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The reaction of guanine residues with dimethylsulfate was studied for complexes of recA protein with fluorescent dye tagged double stranded oligonucleotides. The patterns of dimethylsulfate modification obtained demonstrate a similarity of DNA states in the complexes with recA protein formed as a result of recA promoted strand exchange and renaturation reactions. The guanine modification efficiency varies periodically as a function of the base position along the oligonucleotide axis, with a period of 3 nucleotides. This effect suggests that the arrangement of recA monomers along the oligonucleotide is strictly ordered, and the dimethylsulfate reactivity of a guanine residue depends on the site of its binding in a recA monomer.

【 授权许可】

Unknown