FEBS Letters | |
Efficient interaction of recA protein with fluorescent dye‐labeled oligonucleotides | |
Bocharova, Tatjana N.1  Volodin, Alexander A.1  Smirnova, Helen A.1  | |
[1] Institute of Molecular Genetics of the Russian Academy of Sciences, Kurchatov Sq., Moscow, 123182, Russian Federation | |
关键词: recA protein; Fluorescent dye-labeled oligonucleotide; recA-oligonucleotide complex; Strand exchange; ATP-S; adenosine-5′-O-(3-thiotriphosphate); JOE; 4′; 5′-dichloro-2′; 7′-dimethoxy-6-carboxy-fluorescein; PAGE; polyacryl-amide gel electrophoresis; ds-; ss-; double- and single-stranded unmodified oligonucleotides respectively; ds*-; ss*-; double- and single stranded dye-labeled oligonucleotides respectively; b.; base; bp; base pair; SDS; dodecyl sulfate sodium salt; | |
DOI : 10.1016/0014-5793(94)00640-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Some fluorescein derivatives attached to the 5′-end of oligonucleotides stimulate recA protein—oligonucleotide binding. The complex formation at near stoichiometric DNA/protein ratios is demonstrated for 18-bases-long oligonucleotides. The complexes with dye-labeled oligonucleotides are shown to be active in the reaction of homologous strand exchange. The strand exchange reaction in the presence of adenosine-5′-O-(3-thiotriphosphate) proceeds with the formation of a stable complex of recA protein with the double stranded oligonucleotide, which is a product of the strand exchange. The displaced single-stranded oligonucleotide is shown to be bound weakly.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020299798ZK.pdf | 325KB | download |