Expression of the VRK (vaccinia‐related kinase) gene family of p53 regulators in murine hematopoietic development

FEBS Letters

Gonzalo, Pilar²  Gaspar, Marı́a L²  Lazo, Pedro A¹  Vega, Francisco M¹ 

[1] Instituto de Biologı́a Molecular y Celular del Cáncer, Centro de Investigación del Cáncer, Consejo Superior de Investigaciones Cientı́ficas, Universidad de Salamanca, E-37007 Salamanca, Spain;Unidad de Inmunobiologı́a, Centro Nacional de Microbiologı́a, Instituto de Salud Carlos III, E-28220 Majadahonda, Spain

关键词: VRK1;    VRK2;    VRK3;    p53;    Ser-Thr kinase;    Mouse development;    Liver;    Hematopoiesis;   

DOI  :  10.1016/S0014-5793(03)00501-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The vaccinia-related kinase (VRK) proteins are a new group of three Ser-Thr kinases in the human kinome. VRK proteins are upstream regulators of several transcription factors. VRK1 phosphorylates p53 in Thr-18 within the region of binding to mdm2 preventing their interaction. The tissue distribution of three genes is still largely unknown. In the present report the expression of these genes was analyzed during murine hematopoietic development. The three genes are expressed in fetal liver and peripheral blood, with higher levels between days 11.5 and 13.5, a time when there is a massive expansion of liver cells, and thereafter their expression falls significantly. VRK genes are expressed, particularly at mid-gestation, in embryo thymus and spleen, but in adult thymus and spleen their levels are very low. VRK2 is expressed at lower levels than VRK1 and VRK3 in the mouse embryo. VRK genes play a role during embryonic development of hematopoiesis.

【 授权许可】

Unknown