An archaeal homing endonuclease I‐PogI cleaves at the insertion site of the neighboring intron, which has no nested open reading frame1

FEBS Letters

Morinaga, Yayoi¹  Sako, Yoshihiko¹  Nomura, Norimichi¹  Uchida, Aritsune¹  Nunoura, Takuro¹  Nakayama, Hitoshi¹ 

[1] Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan

关键词: Mobile intron;    Intron homing;    Homing endonuclease;    LAGLIDADG family;    Flanking marker co-conversion;    Archaea;    CS;    cleavage site;    IS;    insertion site;    rDNA;    rRNA gene;    HE;    homing endonuclease;   

DOI  :  10.1016/S0014-5793(03)00497-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Homing endonucleases (HEs) of the LAGLIDADG family cleave intron/inteinless cognate DNA at, or near, the insertion site (IS) of their own intron/intein. Here, we describe a notable exception to this rule. Two introns, Pog.S1205 (length 32 bp) and Pog.S1213 (664 bp), whose ISs are 8 bp apart, exist within the 16S rRNA gene of the archaeon Pyrobaculum oguniense. Pog.S1213 harbors a nested open reading frame (ORF) encoding a 22 kDa monomeric protein, I-PogI, which contains two LAGLIDADG motifs and has optimal DNA cleavage activity at 90°C. Intriguingly, I-PogI cleaves the Pog.S1205-less substrate DNA in the presence or absence of Pog.S1213. The cleavage site (CS) of I-PogI does not coincide with the IS of Pog.S1213 but with that of Pog.S1205. Thus, I-PogI activity both promotes the homing of its own intron, Pog.S1213, and guarantees co-conversion of the ORF-less intron Pog.S1205.

【 授权许可】

Unknown