FEBS Letters | |
An archaeal homing endonuclease I‐PogI cleaves at the insertion site of the neighboring intron, which has no nested open reading frame1 | |
Morinaga, Yayoi1  Sako, Yoshihiko1  Nomura, Norimichi1  Uchida, Aritsune1  Nunoura, Takuro1  Nakayama, Hitoshi1  | |
[1] Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan | |
关键词: Mobile intron; Intron homing; Homing endonuclease; LAGLIDADG family; Flanking marker co-conversion; Archaea; CS; cleavage site; IS; insertion site; rDNA; rRNA gene; HE; homing endonuclease; | |
DOI : 10.1016/S0014-5793(03)00497-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Homing endonucleases (HEs) of the LAGLIDADG family cleave intron/inteinless cognate DNA at, or near, the insertion site (IS) of their own intron/intein. Here, we describe a notable exception to this rule. Two introns, Pog.S1205 (length 32 bp) and Pog.S1213 (664 bp), whose ISs are 8 bp apart, exist within the 16S rRNA gene of the archaeon Pyrobaculum oguniense. Pog.S1213 harbors a nested open reading frame (ORF) encoding a 22 kDa monomeric protein, I-PogI, which contains two LAGLIDADG motifs and has optimal DNA cleavage activity at 90°C. Intriguingly, I-PogI cleaves the Pog.S1205-less substrate DNA in the presence or absence of Pog.S1213. The cleavage site (CS) of I-PogI does not coincide with the IS of Pog.S1213 but with that of Pog.S1205. Thus, I-PogI activity both promotes the homing of its own intron, Pog.S1213, and guarantees co-conversion of the ORF-less intron Pog.S1205.
【 授权许可】
Unknown
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