FEBS Letters | |
Structural parsimony in endonuclease active sites: should the number of homing endonuclease families be redefined? | |
Hemmings, Andrew M.2  Kühlmann, Ulrike C.2  James, Richard1  Kleanthous, Colin1  Moore, Geoffrey R.2  | |
[1] School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK;School of Chemical Sciences, University of East Anglia, Norwich NR4 7TJ, UK | |
关键词: Colicin E9; Nuclease active site motif; Homing endonuclease; | |
DOI : 10.1016/S0014-5793(99)01499-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Homing endonucleases are classified into four families based on active site sequence motifs. Through structural comparisons we have found structural similarities between the endonuclease domain of colicin E9, an H-N-H motif-containing enzyme, and both the non-specific nuclease from Serratia and I-PpoI, a His-Cys box-containing homing endonuclease. Our comparison identifies conservation at the heart of all three enzyme active sites and so argues for a re-classification of H-N-H and His-Cys box homing endonucleases as a single family. We suggest the ‘ββα-Me family’ of homing enzymes to reflect the three elements of secondary structure and the metal ion that define the motif.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020308703ZK.pdf | 201KB | download |