Early changes in glucose and phospholipid metabolism following apoptosis induction by IFN‐γ/TNF‐α in HT‐29 cells

FEBS Letters

Cozzone, Patrick J¹  Lutz, Norbert W¹  Tome, Margaret E² 

[1] Centre de Résonance Magnétique Biologique et Médicale, Faculté de Médecine, Marseille, France;Department of Pathology, University of Arizona, Tucson, AZ, USA

关键词: Apoptosis;    Cytokine treatment;    HT-29 cells;    Metabolism;    Nuclear magnetic resonance spectroscopy;    Fru-1;    6-DP;    fructose-1;    6-diphosphate;    GPC;    sn-glycero-(3)-phosphocholine;    Gro-3-P;    glycerol-3-phosphate;    IFN;    interferon;    LDH;    lactate dehydrogenase (EC 1.1.1.27);    NTP;    all nucleoside 5’-triphosphates;    PC;    phosphocholine;    TNF;    tumor necrosis factor;    tot. P;    total 31P signal integral;   

DOI  :  10.1016/S0014-5793(03)00489-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of apoptosis induction on glucose and phospholipid metabolite levels in cancer were studied using human colon adenocarcinoma cells (HT-29). Apoptosis was induced by co-incubation with 200 U/ml tumor necrosis factor (TNF)-α for 4, 8 or 15 h, after sensitization with 500 U/ml interferon (IFN)-γ for 7 h. Perchloric acid extracts were analyzed by ¹H and ³¹P nuclear magnetic resonance (NMR) spectroscopy. Significantly increased lactate and NTP (all nucleoside 5’-triphosphates) signals were detected 4 h after apoptosis-inducing IFN-γ/TNF-α treatment, but not in cells which were TNF-α-treated without IFN-γ preincubation. Simultaneous lactate and NTP changes, if confirmed in vivo, may serve as early, non-invasive markers of treatment response in some tumors.

【 授权许可】

Unknown