Microbial isoprenoid biosynthesis and human γδ T cell activation

FEBS Letters

Eberl, Matthias¹  Kollas, Ann-Kristin¹  Reichenberg, Armin¹  Hintz, Martin¹  Wiesner, Jochen¹  Jomaa, Hassan¹ 

[1] Biochemisches Institut, Justus-Liebig-Universität Giessen, Friedrichstr. 24, 35392 Giessen, Germany

关键词: GcpE;    LytB;    DOXP;    MEP;    HMB-PP;    IPP;    DMAPP;    dimethylallyl pyrophosphate;    DOXP;    1-deoxy-D-xylulose 5-phosphate;    Dxr;    1-deoxy-D-xylulose 5-phosphate reductoisomerase;    Dxs;    1-deoxy-D-xylulose 5-phosphate synthase;    HMB-PP;    (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate;    HMG-CoA;    3-hydroxy-3-methylglutaryl-CoA;    IPP;    isopentenyl pyrophosphate;    MEcPP;    2-C-methyl-D-erythritol 2;    4-cyclopyrophosphate;    MEP;    2-C-methyl-D-erythritol 4-phosphate;   

DOI  :  10.1016/S0014-5793(03)00483-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human Vγ9/Vδ2 T cells play a crucial role in the immune response to microbial pathogens, yet their unconventional reactivity towards non-peptide antigens has been enigmatic until recently. The break-through in identification of the specific activator was only possible due to recent success in a seemingly remote field: the elucidation of the reaction steps of the newly discovered 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway of isoprenoid biosynthesis that is utilised by many pathogenic bacteria. Unexpectedly, the intermediate of the MEP pathway, (E)-4-hydroxy-3-methyl-but-2-enyl-pyrophosphate) (HMB-PP), turned out to be by far the most potent Vγ9/Vδ2 T cell activator known, with an EC₅₀ of 0.1 nM.

【 授权许可】

Unknown