The Drosophila homolog of Aut1 is essential for autophagy and development

FEBS Letters

Sinka, Rita¹  Juhász, Gábor²  Csikós, György²  Erdélyi, Miklós¹  Sass, Miklós² 

[1]Biological Research Center, H-6701 Temesvári krt. 62, Szeged, Hungary

[2]Department of General Zoology, Eötvös Loránd University, H-1117 Pázmány sétány 1/C, Budapest, Hungary

关键词: Drosophila;    Autophagy;    Fat body;    Aut1/Apg3;    CG6877/Draut1;    RNA interference;    EST;    expressed sequence tag;    PAGE;    polyacrylamide gel electrophoresis;    Draut1p;    Draut1 protein;    20E;    20-hydroxy-ecdysone;    RNAi;    RNA interference;    UAS;    upstream activating sequence;    EIR;    expression of an inverted repeat;   

DOI  :  10.1016/S0014-5793(03)00431-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Drosophila homolog of yeast Aut1, CG6877/Draut1, is a ubiquitously expressed cytosolic protein. Draut1 loss of function was achieved by expression of an inverted repeat transgene inducing RNA interference. The effect is temperature-dependent and resembles an allelic series as described by Fortier, E. and Belote, J.M. (Genesis 26 (2000) 240–244). Draut1 loss of function larvae are unable to induce autophagy and heterophagy in fat body cells before pupariation and die during metamorphosis. To our knowledge, this is the first report of a multicellular animal lacking the function of a gene participating in the protein conjugation systems of autophagy.

【 授权许可】

Unknown