Microwave radiation can alter protein conformation without bulk heating

FEBS Letters

Candido, E.Peter M.¹  Smith, Tim²  Dawe, Adam²  Jones, Donald¹  Smith, Brette²  de Pomerai, David I.²  Archer, David B.²  North, Kate²  Duce, Ian R.² 

[1] Department of Biochemistry and Molecular Biology, 2146 Health Sciences Mall, University of British Columbia, Vancouver, BC, Canada V6T 1Z3;School of Life and Environmental Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK

关键词: RNA interference;    Microwave radiation;    Non-thermal;    Protein aggregation;    Amyloid fibril;    Caenorhabditis elegans;   

DOI  :  10.1016/S0014-5793(03)00413-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exposure to microwave radiation enhances the aggregation of bovine serum albumin in vitro in a time- and temperature-dependent manner. Microwave radiation also promotes amyloid fibril formation by bovine insulin at 60°C. These alterations in protein conformation are not accompanied by measurable temperature changes, consistent with estimates from field modelling of the specific absorbed radiation (15–20 mW kg⁻¹). Limited denaturation of cellular proteins could explain our previous observation that modest heat-shock responses are induced by microwave exposure in Caenorhabditis elegans. We also show that heat-shock responses both to heat and microwaves are suppressed after RNA interference ablating heat-shock factor function.

【 授权许可】

Unknown