Retinoic acid specifically activates an oleate‐dependent phospholipase D in the nuclei of LA‐N‐1 neuroblastoma cells

FEBS Letters

Antony, Pierre²  Kanfer, Julian N¹  Freysz, Louis² 

[1] Department of Chemistry, Stetson University, DeLand, FL 32720-3756, USA;Institut de Chimie Biologique, Faculte de Medecine, Laboratoire de Neurobiologie Moleculaire des Interactions Cellulaires, 11 rue Humann, 67085 Strasbourg Cedex, France

关键词: Phospholipase D;    LA-N-1 nucleus;    Retinoic acid;    Retinoic acid receptor;    Cellular differentiation;    PLC;    phospholipase C;    PLD;    phospholipase D;    PtdCho;    phosphatidylcholine;    PtdEtOH;    phosphatidylethanol;    DAG;    diacylglycerol;    PtdOH;    phosphatidic acid;    RA;    retinoic acid;    atRA;    all-trans-retinoic acid;   

DOI  :  10.1016/S0014-5793(03)00313-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Earlier studies showed that treatment of LA-N-1 cells with TPA, a tumoral promoter, leads to the stimulation of a G protein-regulated phospholipase D (PLD) in the nuclei. Now we demonstrate that retinoic acid, a cellular differentiation inducing agent, activates a nuclear oleate-dependent PLD in LA-N-1 cells. Treatment of the nuclei with retinoic acid induces the breakdown of phosphatidylcholine (PtdCho). Our results indicate that PLD is regulated differentially depending on the nature of the stimulatory agent. These results strongly suggest the existence of two nuclear PLD isoforms in LA-N-1 nuclei that hydrolyze PtdCho.

【 授权许可】

Unknown