Comparison of the anti‐apoptotic effects of Bcr‐Abl, Bcl‐2 and Bcl‐xL following diverse apoptogenic stimuli

FEBS Letters

Brumatti, Gabriela¹  Amarante-Mendes, Gustavo P¹  Yon, Monica¹  Chehab, Cristina F¹  Weinlich, Ricardo¹ 

[1] Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, and Institute for Investigation in Immunology, Millenium Institute, Av. Prof. Lineu Prestes, 1730 Cidade Universitária, São Paulo, SP 05508-900, Brazil

关键词: Apoptosis;    Bcr-Abl;    Bcl-2;    Bcl-xL;    Mitochondrion;    Act-D;    actinomycin D;    Ara-C;    cytosine arabinoside;    Cal-C;    calphostin C;    CHX;    cycloheximide;    mAb;    monoclonal antibody;    Noco;    nocodazole;    pAb;    polyclonal antibody;    STS;    staurosporine;    VCS;    vincristine sulfate;    VP-16;    etoposide;   

DOI  :  10.1016/S0014-5793(03)00299-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ectopic expression of Bcr-Abl, Bcl-2 or Bcl-x_L in HL-60 cells conferred resistance to apoptosis against a variety of death-inducing agents. Bcr-Abl-mediated interference with mitochondrial events was confirmed by the analysis of the loss of mitochondrial transmembrane potential and cytochrome c release. HL-60.Bcr-Abl cells were extremely resistant to all apoptogenic stimuli tested, even in circumstances where HL-60.Bcl-2 or HL-60.Bcl-x_L cells were only partially protected from apoptosis. The levels of Mcl-1, Bax, Bid, Akt, c-IAP-1, c-IAP-2, XIAP and c-FLIP were compared in all HL-60 lines. Our findings show that Bcr-Abl is a more powerful anti-apoptotic molecule than Bcl-2 or Bcl-x_L.

【 授权许可】

Unknown