Disabled‐2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells

FEBS Letters

Tseng, Chin-Hsiao³  Cheng, Ju-Chien⁵  Huang, Chien-Ling¹  Huang, Ching-Hui⁴  Tseng, Ching-Ping¹  Chiu, Daniel Tsun-Yee¹  Stern, Arnold² 

[1] Graduate Institute of Medical Biotechnology Chang Gung University, 259 Wen-Hwa 1 Road, Kweishan, Taoyuan 333, Taiwan;Department of Pharmacology, New York University School of Medicine, New York, NY, USA;Department of Internal Medicine, National Taiwan University, Taipei, Taiwan;Graduate Institute of Basic Medical Sciences, Chang Gung University, Taoyuan, Taiwan;Department of Medical Technology, China Medical College, Taichung, Taiwan

关键词: Disabled-2;    Small interfering RNA;    K562 cell;    Megakaryocytic differentiation;    Mitogen-activated protein kinase;   

DOI  :  10.1016/S0014-5793(03)00281-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previous studies have shown that Disabled-2 (DAB2) is up-regulated during megakaryocytic differentiation of human K562 cells. To delineate the consequences of DAB2 induction, a DNA vector-based small interfering RNA (siRNA) was designed to intervene in DAB2 expression. We found that DAB2 siRNA specifically inhibited DAB2 induction, resulting in the modulation of cell–cell adhesion and mitogen-activated protein kinase (MAPK) phosphorylation. The morphological changes and β3 integrin expression associated with megakaryocytic differentiation were not affected. Since the MAPK pathway has been shown to involve DAB2 induction [Tseng et al., Biochem. Biophys. Res. Commun. 285 (2001) 129–135], our results suggest a reciprocal regulation between DAB2 and MAPK in the differentiation of K562 cells. In addition, we have demonstrated for the first time that DAB2 siRNA is a valuable tool for unveiling the biological consequences of DAB2 expression.

【 授权许可】

Unknown