FEBS Letters | |
Disabled‐2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells | |
Tseng, Chin-Hsiao3  Cheng, Ju-Chien5  Huang, Chien-Ling1  Huang, Ching-Hui4  Tseng, Ching-Ping1  Chiu, Daniel Tsun-Yee1  Stern, Arnold2  | |
[1] Graduate Institute of Medical Biotechnology Chang Gung University, 259 Wen-Hwa 1 Road, Kweishan, Taoyuan 333, Taiwan;Department of Pharmacology, New York University School of Medicine, New York, NY, USA;Department of Internal Medicine, National Taiwan University, Taipei, Taiwan;Graduate Institute of Basic Medical Sciences, Chang Gung University, Taoyuan, Taiwan;Department of Medical Technology, China Medical College, Taichung, Taiwan | |
关键词: Disabled-2; Small interfering RNA; K562 cell; Megakaryocytic differentiation; Mitogen-activated protein kinase; | |
DOI : 10.1016/S0014-5793(03)00281-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Previous studies have shown that Disabled-2 (DAB2) is up-regulated during megakaryocytic differentiation of human K562 cells. To delineate the consequences of DAB2 induction, a DNA vector-based small interfering RNA (siRNA) was designed to intervene in DAB2 expression. We found that DAB2 siRNA specifically inhibited DAB2 induction, resulting in the modulation of cell–cell adhesion and mitogen-activated protein kinase (MAPK) phosphorylation. The morphological changes and β3 integrin expression associated with megakaryocytic differentiation were not affected. Since the MAPK pathway has been shown to involve DAB2 induction [Tseng et al., Biochem. Biophys. Res. Commun. 285 (2001) 129–135], our results suggest a reciprocal regulation between DAB2 and MAPK in the differentiation of K562 cells. In addition, we have demonstrated for the first time that DAB2 siRNA is a valuable tool for unveiling the biological consequences of DAB2 expression.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020312881ZK.pdf | 403KB | download |