Interaction between cationic agents and small interfering RNA and DNA molecules

18th International Conference PhysicA.SPb

Unksov, I.N.^1 ; Slita, A.V.^2 ; Petrova, A.V.^2,3 ; Pereviazko, I.^1 ; Bakulev, V.M.^1 ; Rolich, V.I.^1 ; Bondarenko, A.B.^1,2 ; Kasyanenko, N.A.^1

St.Petersburg State University, Petrodvorets, Uluanovskaya str., 1, St.-Petersburg

198504, Russia^1

Research Insitute of Influenza, Prof. Popova str., 15/17, St.-Petersburg

197022, Russia^2

Peter the Great St.Petersburg Polytechnic University, Polytechnicheskaya str., 29, St.-Petersburg

195251, Russia^3

关键词: Cationic agents;    DNA molecules;    Flow birefringence;    In-vitro;    Small interfering RNA;    Spectral methods;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/769/1/012023/pdf
DOI  :  10.1088/1742-6596/769/1/012023

来源: IOP

【 摘 要 】

Azobenzene containing surfactant AzoTAB was used for investigation of binding in cationic- agent + nucleic acid in NaCl salt aqueous solutions. Two nucleic acids, macromolecular DNA and small interfering RNA, were examined upon the interaction with the surfactant. For DNA the interaction was studied using spectral methods and the methods of viscometry and flow birefringence measurement. For siRNA the possibility of surfactant-based delivery was checked in vitro.