PCR‐linked in vitro expression: a novel system for high‐throughput construction and screening of protein libraries

FEBS Letters

Nakano, Hideo¹  Rungpragayphan, Suang¹  Yamane, Tsuneo¹ 

[1] Laboratory of Molecular Biotechnology, Graduate School of Biological and Agricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan

关键词: Single-molecule polymerase chain reaction;    In vitro coupled transcription/translation;    Combinatorial protein library;    High-throughput screening;    SM-PCR;    single-molecule polymerase chain reaction;    SIMPLEX;    single-molecule-PCR-linked in vitro expression;    anti-HSA-scFv;    anti-human serum albumin single chain variable fragment;   

DOI  :  10.1016/S0014-5793(03)00251-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel entirely in vitro strategy for generation and screening of a combinatorial protein library in an array format has been developed and is experimentally demonstrated. The strategy exploits virtues of PCR and in vitro coupled transcription/translation. Our new approach provides high-throughput construction and screening of the in vitro protein library, and compatibility with various selection methods.

【 授权许可】

Unknown