Targeting of MIST to Src‐family kinases via SKAP55–SLAP‐130 adaptor complex in mast cells

FEBS Letters

Komurasaki, Toshi²  Hozumi, Nobumichi⁴  Wakahara, Shunichi²  Tatsuno, Akiko⁴  Fujii, Yasuyuki²  Ohtake, Hidenori²  Kitamura, Daisuke⁴  Ra, Chisei³  Nakao, Toru¹  Kitamura, Kunihiro¹  Fujiwara, Naruyoshi⁴  Goitsuka, Ryo⁴  Hara, Toshifumi² 

[1] Ethical Business Strategy Division, Medicinal Research Laboratories, Taisho Pharmaceutical Co. Ltd., 1-403 Yoshino-cho, Saitama-shi, Saitama 330-8530, Japan;Department of Molecular Biology Laboratory, Medicinal Research Laboratories, Taisho Pharmaceutical Co. Ltd., 1-403 Yoshino-cho, Saitama-shi, Saitama 330-8530, Japan;Department of Molecular Cell Immunology and Allergology, Advanced Medical Research Center, Nihon University School of Medicine, Tokyo, Japan;Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan

关键词: Signal transduction;    Protein tyrosine kinase;    Mast cell;    IgE receptor;    Adaptor;    Ab;    antibody;    FcϵRI;    Fc receptor type I for IgE;    GST;    glutathione S-transferase;    ITAM;    immunoreceptor tyrosine-based activation motif;    MAPK;    mitogen-activated protein kinase;    PLC;    phospholipase C;    PR;    proline-rich;    PTK;    protein tyrosine kinase;    SH;    Src homology;   

DOI  :  10.1016/S0014-5793(03)00234-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

MIST (mast cell immunoreceptor signal transducer; also termed Clnk) is an adaptor protein structurally related to SLP-76-family hematopoietic cell-specific adaptor proteins. We demonstrate here that two major MIST-associated phosphoproteins expressed in mast cell lines are SLAP-130 and SKAP55, adaptors known to interact with the Src-homology (SH) 2 domain of Src-family protein tyrosine kinases (PTKs). MIST directly associated with SLAP-130 via its SH2 domain, and collaboration of SLAP-130 with SKAP55 was required for the recruitment of MIST to Lyn. Furthermore, MIST was preferentially recruited to Fyn rather than Lyn, which is regulated by higher affinity binding of SLAP-130 and SKAP55 with the Fyn-SH2 domain than the Lyn-SH2 domain. Our results suggest that the MIST–SLAP-130–SKAP55 adaptor complex functions downstream of high-affinity IgE receptor-associated Src-PTKs in mast cells.

【 授权许可】

Unknown