Mouse monoclonal antibodies that specifically recognize an amino‐terminal epitope of p56lck protein tyrosine kinase

FEBS Letters

Jacot-Guillermod, Hubert¹  Burn, Paul¹  Stieger, Martin¹  Flint, Nicholas A.¹ 

[1] Department of Biology, Pharmaceutical Research-New Technologies, F. Hoffmann-La Roche Ltd., 4002 Basel, Switzerland

关键词: Protein tyrosine kinase;    Monoclonal antibody;    p56lck;    Epitope mapping;    Signal transduction;   

DOI  :  10.1016/0014-5793(93)80617-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

p56^lck is a T cell-specific protein tyrosine kinase of the src family of proto-oncogenes which has been implicated in T cell signal transduction. Here we describe the production of mouse monoclonal antibodies directed against recombinant human p56^lck purified from an E. coli expression system. The antibodies were characterized by ELISA, RIA and immunoprecipitation of p56^lck from T cell lysates. A specific epitope was revealed at the aminoterminus of the p56^lck molecule by using Western blotting of deletion mutants and distinct domains of p56^lck expressed in E. coli. Potential applications of the results obtained are discussed.

【 授权许可】

Unknown