The p110γ PI‐3 kinase is required for EphA8‐stimulated cell migration

FEBS Letters

Gu, Changkyu²  Park, Soochul¹ 

[1] Department of Life Science, Sookmyung Women's University, 53-12 Chungpa-Dong 2-Ka, Yongsan-Ku, Seoul 140-742, South Korea;Institute of Natural Science, Sookmyung Women's University, 53-12 Chungpa-Dong 2-Ka, Yongsan-Ku, Seoul 140-742, South Korea

关键词: Eph;    Ephrin;    p110γ PI-3 kinase;   

DOI  :  10.1016/S0014-5793(03)00223-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study provides evidence that treatment with preclustered ephrin A5-Fc results in a substantial increase in the stability of the p110γ PI-3 kinase associated with EphA8, thereby enhancing PI-3 kinase activity and cell migration on a fibronectin substrate. In contrast, co-expression of a lipid kinase-inactive p110γ mutant together with EphA8 inhibits ligand-stimulated PI-3 kinase activity and cell migration on a fibronectin substrate, suggesting that the mutant has a dominant negative effect against the endogenous p110γ PI-3 kinase. Significantly, the tyrosine kinase activity of EphA8 is not important for either of these processes. Taken together, our results demonstrate that the stimulation of cell migration on a fibronectin substrate by the EphA8 receptor depends on the p110γ PI-3 kinase but is independent of a tyrosine kinase activity.

【 授权许可】

Unknown