FEBS Letters | |
The p110γ PI‐3 kinase is required for EphA8‐stimulated cell migration | |
Gu, Changkyu2  Park, Soochul1  | |
[1] Department of Life Science, Sookmyung Women's University, 53-12 Chungpa-Dong 2-Ka, Yongsan-Ku, Seoul 140-742, South Korea;Institute of Natural Science, Sookmyung Women's University, 53-12 Chungpa-Dong 2-Ka, Yongsan-Ku, Seoul 140-742, South Korea | |
关键词: Eph; Ephrin; p110γ PI-3 kinase; | |
DOI : 10.1016/S0014-5793(03)00223-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
This study provides evidence that treatment with preclustered ephrin A5-Fc results in a substantial increase in the stability of the p110γ PI-3 kinase associated with EphA8, thereby enhancing PI-3 kinase activity and cell migration on a fibronectin substrate. In contrast, co-expression of a lipid kinase-inactive p110γ mutant together with EphA8 inhibits ligand-stimulated PI-3 kinase activity and cell migration on a fibronectin substrate, suggesting that the mutant has a dominant negative effect against the endogenous p110γ PI-3 kinase. Significantly, the tyrosine kinase activity of EphA8 is not important for either of these processes. Taken together, our results demonstrate that the stimulation of cell migration on a fibronectin substrate by the EphA8 receptor depends on the p110γ PI-3 kinase but is independent of a tyrosine kinase activity.
【 授权许可】
Unknown
【 预 览 】
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